Recombinant expression and development of enzyme-based detoxification strategies for OP-based pesticides

Abstract

Pesticides are widely used in agriculture to protect crops from pests and increase yields. However, their use has been associated with adverse environmental and health effects due to organophosphate (OP) pesticides. OP poisoning has been linked to various symptoms, including neurological disorders and death. To address these issues, there is a growing interest in developing methods for detecting pesticides and the remediation of contaminated soils and water. The present study investigates the enzymatic degradation of various OP Pesticides. Investigating the use of immobilized enzymes in bioremediation processes for the degradation of OP pesticides and the real-life application of enzymes degrading OP-based Pesticides. The research involved discovering a new substrate being degraded by OP hydrolyzing enzymes. The enzyme was also immobilized onto alginate beads. These immobilized enzymes were then tested for their ability to degrade OP pesticides in contaminated water samples. The results showed that the immobilized enzymes effectively degraded OP pesticides, with degradation rates up to 99% using 0.2U of immobilized enzyme. The efficiency of the immobilized enzymes was found to be dependent on several factors, including the type of enzyme, the immobilization method, and the characteristics of the contaminated environment. The research also evaluated the stability and reusability of the immobilized enzymes and found that they retained their activity after several cycles of use. Overall, the findings of this study suggest that immobilized enzymes can be a promising tool for the bioremediation of OP pesticide contaminated environments offering several advantages, including increased stability and reusability, and can provide an environmentally friendly and cost-effective approach for removing OP pesticides. The research also highlights the need to optimize further and develop immobilized enzyme-based bioremediation strategies to clean contaminated environments effectively.