Antisense oligonucleotide delivery

Abstract

PNAs have gained significant attention in the realms of therapeutics, diagnostics, and biomedical research due to their versatility for targeted DNA sequence recognition. This research report presents a synthetic approach to develop alanyl-PNAs as DNA mimics for precise gene expression modulation. Our methodology involves the design and synthesis of a fundamental alanyl-PNA monomeric unit, starting from the amino acid (L-phenylalanine) and incorporating it into a serine building block to obtain a key intermediate which ultimately goes via a substitution pathway to have nucleobase-containing peptide nucleic acid. We accomplished these essential steps using solution-phase peptide synthesis, forming a crucial part of developing the alanyl-PNA which we aimed to develop.