Design and construction of fluorescent bioactive probes for bioimaging of targeted cellular organelles

Abstract

The work demonstrated in thesis entitled “DESIGN AND CONSTRUCTION OF FLUORESCENT BIOACTIVE PROBES FOR BIOIMAGING OF TARGETED CELLULAR ORGANELLES” was initiated in December 2014 in the Discipline of Biosciences and Biomedical Engineering (BSBE), Indian Institute of Technology Indore. The fluorescence imaging technique has emerged as a powerful way to monitor the amount, localization and movement of biomolecules at the cellular level. The objectives of this thesis are to design and synthesis of various organelles targeting fluorescent probes. The focal points of thesis are as follows:- 1. Design and synthesis and characterization of fluorescent organelles targeting probes. 2. Synthesis of two photon probes for their advance application in bio-imaging and deeper tissue penetration capability. 3. Live cell/tumor spheroid bio-imaging using synthesized probe and compared with standard reference trackers for validating cellular organelles targeting ability of probes. 4. Solid state properties of single crystal explored in terms of unique non-bonding interaction and elastic bending, followed by organelles bio-imaging ability in solution state. 5. Synthesis of biologically active Aroyl-hydrazone based ligands and determines their effectiveness by molecular docking, density function calculation (DFT), DNA and proteins binding.