In silico analysis, cloning and recombinant expression of membrane proteins from Salmonella and H. pylori

Abstract

Gram-negative bacterial infections pose a massive risk for the safety of humans all around the globe. With increasing resistance to almost every known antibiotic, these organisms are getting harder to deal with each day. There is a dire need to pay close attention to the molecular machinery these pathogens involve and use them for our benefit. This can help to effectively eliminate them using more potent therapeutics and provide insights on how effectively the GNB can evade the harmful conditions implicated in them. This thesis aims to cover and study Salmonella (IMP) and H. pylori (HefD) membrane proteins to figure out if these proteins are crucial for their cellular integrity, growth, and survival. Firstly, the in-silico structural and functional characterizations are performed to understand their functions and roles. It is followed by cloning these proteins in expression vectors in order to perform wet-lab structure and function determination experiments. Keywords: Salmonella, H. pylori, Gram-negative, membrane proteins, efflux pumps.