Quality assessment of medicinal leaves through biospeckle technique

Abstract

In this paper, we report investigations undertaken towards visualization of microbiological objects in medicinal leaves using biospeckle activity approach. Both fresh and infected samples of Ficus Religiosa (peepal leaf) are illuminated by a laser source for generation of biospeckle pattern. Dynamic variation in activity images were grabbed by a Charge Couple Device (CCD) camera and quantized into 8 bit digital image using frame grabber. For image analysis revised version of conventional Fujii’s method i.e. weighted parameterized Fujii method is used. It shows high contrast as compared to the other existing methods when sample is illuminated uniformly. The current study aims to distinguish between fresh and infected leaves for improvement of quality of Ayurvedic medicines. © 2017, Springer Nature Singapore Pte Ltd.