Synthesis, characterization and properties of a water soluble, naphthyl monoimide based unnatural amino acid and its binding studies with DNA

Abstract

Fluorescence molecules with long emission region and high intensity are very important to detect biomolecules. Deoxyribonucleic acid (DNA) is one of the most important biomolecule in our body. Small molecules binding with DNA can be used as several biological applications, such as treatment of cancer, parasitic diseases and infections. Small organic molecules binding with DNA in water medium and physiological conditions are very rare. Our aim of this project is to synthesize such molecule which can act as fluorophore, can bind or interact with DNA and can also be soluble in water. 1,8- naphthalic based molecules are important class of DNA binder. Another important thing of these types of molecule is they have high cytotoxicity ability. We can make it fluophore compound from this moiety. In this project, we have designed and synthesized a 1,8- naphthalic monoimide based unnatural amino acid; which is effectively binds with calf-thymus DNA (ct-DNA) at pH 7.4.