Please use this identifier to cite or link to this item: https://dspace.iiti.ac.in/handle/123456789/3999
Title: Structural switching electrochemical DNA aptasensor for the rapid diagnosis of tuberculous meningitis
Authors: Mishra, Subodh Kumar
Kumar, Amit
Keywords: alpha crystallin;methylene blue;single stranded DNA;aptamer;bacterial antigen;bacterial DNA;bacterial protein;HspX protein, Mycobacterium tuberculosis;antigen detection;Article;cerebrospinal fluid analysis;clinical article;diagnostic test accuracy study;diagnostic value;differential pulse voltammetry;gene switching;genetic procedures;human;molecular recognition;sensitivity and specificity;tuberculous meningitis;cerebrospinal fluid;chemistry;diagnostic test;electrochemical analysis;genetics;immunology;isolation and purification;microbiology;Mycobacterium tuberculosis;procedures;tuberculous meningitis;Antigens, Bacterial;Aptamers, Nucleotide;Bacterial Proteins;Biosensing Techniques;Diagnostic Tests, Routine;DNA, Bacterial;Electrochemical Techniques;Humans;Mycobacterium tuberculosis;Tuberculosis, Meningeal
Issue Date: 2019
Publisher: Dove Medical Press Ltd.
Citation: Das, R., Dhiman, A., Mishra, S. K., Haldar, S., Sharma, N., Bansal, A., . . . Sharma, T. K. (2019). Structural switching electrochemical DNA aptasensor for the rapid diagnosis of tuberculous meningitis. International Journal of Nanomedicine, 14, 2103-2113. doi:10.2147/IJN.S189127
Abstract: Background: Tuberculous meningitis (TBM) is the most devastating manifestation of extrapulmonary tuberculosis. About 33% of TBM patients die due to very late diagnosis of the disease. Conventional diagnostic methods based on signs and symptoms, cerebrospinal fluid (CSF) smear microscopy or liquid culture suffer from either poor sensitivity or long turnaround time (up to 8 weeks). Therefore, in order to manage the disease efficiently, there is an urgent and unmet need for a rapid and reliable diagnostic test. Methods: In the current study, to address the diagnostic challenge of TBM, a highly rapid and sensitive structural switching electrochemical aptasensor was developed by combining the electrochemical property of methylene blue (MB) with the molecular recognition ability of a ssDNA aptamer. To demonstrate the clinical diagnostic utility of the developed aptasensor, a blinded study was performed on 81 archived CSF specimens using differential pulse voltammetry. Results: The electrochemical aptasensor developed in the current study can detect as low as 10 pg HspX in CSF background and yields a highly discriminatory response (P<0.0001) for TBM and not-TBM categories with ~95% sensitivity and ~97.5% specificity and has the ability to deliver sample-to-answer in ≤30 minutes. Conclusion: In summary, we demonstrate a new aptamer-based electrochemical biosensing strategy by exploiting the target-induced structural switching of H63 SL-2 M6 aptamer and electroactivity of aptamer-tagged MB for the detection of HspX in CSF samples for the diagnosis of TBM. Further, the clinical utility of this sensor could be extended for the diagnosis of other forms of tuberculosis in the near future. © 2019 Das et al.
URI: https://doi.org/10.2147/IJN.S189127
https://dspace.iiti.ac.in/handle/123456789/3999
ISSN: 1176-9114
Type of Material: Journal Article
Appears in Collections:Department of Biosciences and Biomedical Engineering

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